A protocol for direct somatic embryogenesis of Protea cynaroides L. using zygotic embryos and cotyledon tissues
نویسندگان
چکیده
منابع مشابه
Zygotic embryogenesis versus somatic embryogenesis
This review will summarize molecular and genetic analyses aimed at identifying the mechanisms underlying the sequence of events during plant zygotic embryogenesis. These events are being studied in parallel with the histological and morphological analyses of somatic embryogenesis. The strength and limitations of somatic embryogenesis as a model system will be discussed briefly. The formation of...
متن کاملEMBRYOGENESIS AND RHIZOGENESIS IN MATURE ZYGOTIC EMBRYOS OF OLIVE (OLEA EUROPAEA L.) CULTIVARS MISSION AND KRONEIKI
Organogenesis and neoformant plantlet production were studied by culturing the different parts of mature embryo including radicle and proximal and distal parts of cotyledon of Mission and Kroneiki cultivars. Different parts of embryos were cultures in OMc medium containing growth regulators, such as indole butyric acid (IBA) and 2-isopentenyl adenine (2-ip) or IBA and naphthalene acetic acid (N...
متن کاملProteomic Analysis of Immature Fraxinus mandshurica Cotyledon Tissues during Somatic Embryogenesis: Effects of Explant Browning on Somatic Embryogenesis
Manchurian ash (Fraxinus mandshurica Rupr.) is a valuable hardwood species in Northeast China. In cultures of F. mandshurica, somatic embryos were produced mainly on browned explants. Therefore, we studied the mechanism of explant browning and its relationship with somatic embryogenesis (SE). We used explants derived from F. mandshurica immature zygotic embryo cotyledons as materials. Proteins ...
متن کاملSomatic Versus Zygotic Embryogenesis: Learning from Seeds.
Plant embryogenesis is a fascinating developmental program that is very successfully established in nature in seeds. In case of in vitro somatic embryogenesis this process is subjected to several limitations such as asynchronous differentiation and further development of somatic embryos, malformations and disturbed polarity, precocious germination, lack of maturity, early loss of embryogenic po...
متن کاملCallose Deposition Is Required for Somatic Embryogenesis in Plasmolyzed Eleutherococcus senticosus Zygotic Embryos
Dynamic changes in callose content, which is deposited as a plant defense response to physiological changes, were analyzed during somatic embryogenesis in Eleutherococcus senticosus zygotic embryos plasmolyzed in 1.0 M mannitol. During plasmolysis, callose deposition was clearly observed inside the plasma membrane of zygotic embryo epidermal cells using confocal laser scanning microscopy. The c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Cell, Tissue and Organ Culture
سال: 2007
ISSN: 0167-6857,1573-5044
DOI: 10.1007/s11240-007-9242-3